Library

Microorganism Enzyme Main carbon substrate Minimal media Source Reference pH Temp [°C] Ks [g/L] µmax [1/h] Yx/s [g/g]
Escherichia
coli
HB101
Lipase Glycerol Yes MediaDB 7.0 37.0 0.82
Escherichia
coli
HB101
Lipase D-Mannose Yes MediaDB 7.0 37.0 0.98
Escherichia
coli
HB101
Lipase Acetate Yes MediaDB 7.0 37.0 0.4
Escherichia
coli
HB101
Lipase (S)-Malate Yes MediaDB 7.0 37.0 0.46
Escherichia
coli
HB101
Lipase Succinate Yes MediaDB 7.0 37.0 0.38
Escherichia
coli
JM101
Styrene monooxygenase Glucose Yes Original publication 7.1 30.0 0.32
Escherichia
coli
JM101
Xylene monooxygenase Glucose Yes Original publication 7.4 30.0 0.5 0.43 (+/- 0.040)
Hansenula

Methanol Book 0.12
Hansenula
polymorpha
34438 ATCC
D-xylose No Original Paper 3.5 30.0 0.379 0.16 (+/- 0.000)
Hansenula
polymorpha
34438 ATCC
D-xylose No Original Paper 4.5 30.0 0.428 0.15 (+/- 0.000)
Hansenula
polymorpha
34438 ATCC
D-xylose No Original Paper 5.5 30.0 0.409 0.12 (+/- 0.000)
Hansenula
polymorpha
34438 ATCC
D-xylose No Original Paper 6.5 30.0 0.267 0.44 (+/- 0.000)
Komagataella
pastoris
DSMZ 70382
Glycerol Yes MediaDB 5.0 25.0 0.2
Komagataella
phaffii
CBS7435 MutS
Lipase (CALB) Glycerol Yes Original publication 5.0 25.0 0.15 (+/-0.010) 0.59 (+/- 0.060)
Komagataella
phaffii
CBS7435 MutS
Lipase (CALB) Glycerol Yes biotechsim 5.0 30.0 0.184 (+/-0.008)
Komagataella
phaffii
GS115
Horseradish peroxidase Glycerol Yes Original publication 6.0 30.0 0.28 (+/-0.030) 0.45 (+/- 0.020)
Pichia
pastoris
SMD1168H
Glucose Yes Original publication 5.0 30.0 0.28 0.3 (+/- 0.020)
Pichia
pastoris
SMD1168H
Aminopeptidase B (low) Glucose Yes Original publication 5.0 30.0 0.26 0.32 (+/- 0.010)
Pichia
pastoris
SMD1168H
Aminopeptidase B (med) Glucose Yes Original publication 5.0 30.0 0.22 0.34 (+/- 0.010)
Pichia
pastoris
SMD1168H
Aminopeptidase B (high) Glucose Yes Original publication 5.0 30.0 0.16 0.35 (+/- 0.020)